Research Keywords 【 display / non-display

  • Echinoderm, cell differentiation, biomineralization, gravity, chemical effect for morphogenesis

Research Pursuits 【 display / non-display

  • I am studying on the development and reproduction of echinoids. The sea urchin egg has a polarity, many kind of cells forming skeleton, muscle and digestive tract appear from the one side of egg (vegetal pole). It is well known that the cell line of skeletogenesis is a first group whose cell fate is decided at first. We have examined the developmental potential of each part in vegetal side and shown that most of all vegetal side has a potential of skeletogenesis in the culture after a separation to a small volume. It is considered that the skeletogenic potential is suppressed and each part is regulated to the normal cell fate in embryogenesis.
    Some of metal ions and chemicals have an effect on the morphogenesis of digestive tract. Exogastrula induced by Estrogen or Li, in which the digestive tract project to the outside, is examined. The roles of steroid hormones, such as estrogens, are also examined on the yolk protein production and gametogenesis.

Educational Pursuits 【 display / non-display

  • I teach the lectures and the experimental courses on the systematic zoology and developmental biology. In the lecture of systematic zoology, the characters of body plan of each phylum are expounded in the order of phylogeny. In the lecture of developmental biology, the morphological changes from a fertilized egg to the complete animal body are explained and the controlling mechanism by a interaction between tissues and cells and a signaling pathway of molecules and genes are expounded. Each laboratory courses are in marine laboratory (Marine and Coastal Research Center, Tateyama). In the laboratory course of systematic zoology, students go to sea shore to collect animals and take a boat to collect marine plankton by net. They understand the real animal diversity by examining animals collected by themselves. In the laboratory course of developmental biology, embryo manipulation and immunostaining on the fertilization and early development are performed. I also teach a laboratory course of diving for the observation and research of marine animals.

Future Research Plans, Research Prospects, and Joint Research Potential 【 display / non-display

  • The developmental system on the autonomous ability is investigated by cell culture system of sea urchin. The goal is how to explain such autonomous ability from gene regulation network. Ancient developmental process is remained in the modern species and it is expected to help the understanding the evolution of animal morphogenesis. Also, the experimental system using sea urchin will be provide useful assay system of environmental stress such as chemicals and gravity. The research on the reproduction is directed to the improvement of animal supply as the experimental material.

Degree 【 display / non-display

  • Doctor (Science), 1992/09

Campus Career 【 display / non-display

  • Graduate School of Humanities and Sciences, Research Organization, the Core Section The Natural/Applied Sciences Division, Associate Professor

  • Faculty of Core Research Natural Science Division, Associate Professor

  • Graduate School of Humanities and Sciences, Education Organization, Doctral Program Life Sciences

  • Graduate School of Humanities and Sciences, Education Organization, Doctral Program Life Sciences

  • Graduate School of Humanities and Sciences, Education Organization, Master's Program Life Sciences

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Research Field 【 display / non-display

 

Books 【 display / non-display

  • Methods in Cell Biology. 74

    Blastomere isolation and transplantation., 2004, Sweet H, Amemiya S, Ransick A, Minokawa T, McClay D, Wikramanayake A, Kuraishi R, Kiyomoto M, Nishida H, Henry J, Book, 243-271

  • Developmental Biology Research in Space Advances in Space Biology and Medicine, Vol. 9,

    Morphogenesis and gravity in a whole amphibian embryo and in isolated blastomeres of sea urchins., Elsevier Science, Amsterdam., 2003, Izumi-Kurotani A and Kiyomoto M, Marthy H, Book, 83-99

  • Recent advances in marine biotechnology

    Cellular differentiation in early development of starfish, Oxford and IBH Publishing, 1996, Shirai H, Kiyomoto M, Nagabhushanam, Thompson, Fingerma, Book, 343-367

Papers 【 display / non-display

  • Heterochronic activation of VEGF signaling and the evolution of the skeleton in echinoderm pluteus larvae.

    Evol Dev., 2012.09, Morino Y, Koga H, Tachibana K, Shoguchi E, Kiyomoto M, Wada H., Original, Research paper (scientific journal), Coauthor

  • Par6 regulates skeletogenesis and gut differentiation in sea urchin larvae.

    Dev Genes Evol., 2012.09, Shiomi K, Yamazaki A, Kagawa M, Kiyomoto M, Yamaguchi M, Original, Research paper (scientific journal), Coauthor

  • The culture condition of larval skeletogenic mesenchyme cell for the gravity experiment.

    Space Utilization Research, 2011.01, Kiyomoto M, Izumi-Kurotani A, Eguchi H and Yamaguchi M, Original, Research paper (scientific journal), Capital Author

  • Functional evolution of Ets in echinoderms with focus on the evolution of echinoderm larval skeletons.

    Dev Genes Evol. 220:107-15., 2010.09, Koga H, Matsubara M, Fujitani H, Miyamoto N, Komatsu M, Kiyomoto M, Akasaka K, Wada H., Original, Research paper (scientific journal), Coauthor

  • The culture condition of larval skeletogenic cell for the gravity experiment.

    Space Utilization Research, 2010.01, Kiyomoto M, Izumi-Kurotani A, Eguchi H and Yamaguchi M, Original, Research paper (scientific journal), Capital Author

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Published Article 【 display / non-display

  • The effect of hypergrabity on the spicule formation in the culture of sea urchin micromeres and embryos.

    Space Utilization Research, 2006, Imai M, Izumi-Kurotani A, Eguchi H, Yamaguchi M and Kiyomoto M, Coauthor, Introduction and explanation (scientific journal)

  • The effect of grabity on the spicule formation in the culture of sea urchin micromeres.

    Space Utilization Research, 2005, Imai M, Kiyomoto M, Izumi-Kurotani A, and Eguchi H, Coauthor, Introduction and explanation (scientific journal)

  • The effect of grabity condition for the expression of spicule matrix protein in cultured skeletogenic cells of sea urchin embryo.

    Space Utilization Research, 2004, Kiyomoto M, Izumi-Kurotani A, and Eguchi H, Coauthor, Introduction and explanation (scientific journal)

  • The effect of grabity condition for cultured skeletogenic cells of sea urchin embryo.

    Space Utilization Research, 2003, Kiyomoto M, Izumi-Kurotani A, and Eguchi H, Coauthor, Introduction and explanation (scientific journal)

  • ウニ胚に内在する骨片形成に必要な因子について 

    月刊海洋, 1996, 清本正人, Introduction and explanation (scientific journal)

Presentations 【 display / non-display

  • Development and genetic similarit y in the inbreeding sea urchin He micentrotus puicherrimus

    KIYOMOTO Masato, Domestic, 2015.09, The 86th Annual Meeting of the Zoological Society of Japan, Niigata, The Zoological Society of Japan, Not Invited, Main Speaker

  • Long-term preservation of echinoderm sperm under non-cryo condition for ecotoxicological bioassay

    KIYOMOTO Masato, International, 2014.11, 6th Bilateral Seminar Italy-Japan, Palermo (Italy), National Research Council (Italy), Invited, Main Speaker

  • Preservation of sea urchin egg as a convensional experimental material

    KIYOMOTO Masato, HAMANAKA Gen, HIROSE Mamiko, YAMAGUCHI Mamoru, Domestic, 2014.09, 日本科学教育学会第38回年会, Oomiya, Japan Society for Science Education, Not Invited, Main Speaker

  • Preservation of sea urchin sperm and maintenance of motility

    KIYOMOTO Masato, Domestic, 2014.09, The 85th Annual Meeting of the Zoological Society of Japan, Sendai, The Zoological Society of Japan, Not Invited, Main Speaker

  • Semi-dry sea urchin experiment using preserved egg and sperm supply.

    KIYOMOTO Masato, International, 2014.04, Developmental Biology of the sea urchi XXII, Woods Hole, MA, USA, Developmental Biology of the sea urchi XXII, Not Invited, Main Speaker

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