Research Keywords 【 display / non-display

  • Secondary metabolism, Flavonoid, Anthocyanin, Pigment, Transcription

Research Pursuits 【 display / non-display

  • Anthocyanins and betacyanins, two types of red pigment, have never been found to occur together in plants. Although anthocyanins are widely distributed in higher plants, betacyanins have replaced anthocyanins in the Caryophyllales. The accumulation of flavonols in the Caryophyllales suggests that the step(s) of anthocyanin biosynthesis from dihydroflavonols to anthocyanins could be blocked in the Caryophyllales. So far, anthoacyanidin synthase (ANS) cDNA was isolated from plants of the Caryophyllales and an enzyme activity assay showed that the Caryophyllales possess functional ANS. However, the expression profile revealed that ANS are not expressed in most tissues and organs except the seeds in Spinacia oleracea, suggesting the transcriptional regulation might cause the lack of anthocyanin in the Caryophyllales. Therefore, we are trying to reveal the transcriptioal mechanics of ANS from two aspects of their regulatory elements, cis and trans.
    Transgenic experiments of arabidopusis suggest the lost of function of anthocyanin biosynthetic regulator(s) might suppress anthocyanin occurrence in the Caryophyllales.

Educational Pursuits 【 display / non-display

  • In 2013, I conducted three classes in undergraduate and four classes in graduate courses. The course contents include both basic biology such as plant physiology or plant biochemistry and applied biology for instance metabolic engineering, GMO (gene modified organisms). I have tried to make classroom coursework easier to understand and get my teaching across to all students attending. A doctor, seven master and an undergraduate students have worked in lab under my supervision.

Campus Career 【 display / non-display

  • Graduate School of Humanities and Sciences, Research Organization, the Core Section The Natural/Applied Sciences Division, Professor

  • Faculty of Core Research Natural Science Division, Professor

  • Graduate School of Humanities and Sciences, Education Organization, Doctral Program Life Sciences

  • Graduate School of Humanities and Sciences, Education Organization, Doctral Program Life Sciences

  • Graduate School of Humanities and Sciences, Education Organization, Master's Program Life Sciences

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Research Field 【 display / non-display

 

Books 【 display / non-display

  • バイオサイエンス

    植物バイオテクノロジーと遺伝子組換え作物, オーム社, 2007.05, 作田正明, バイオサイエンス研究会, Textbook

  • 植物分子細胞生物学

    オーム社2004.05, 芦原 坦、作田 正明, Textbook

  • 植物色素研究法

    ベタレイン生成系, 大阪公立大学共同出版会, 2004.03, 作田正明, 植物色素研究会, Research Book

  • 生物学データ大百科事典

    植物の二次代謝, 朝倉書店, 2002.06, 作田正明, 石原勝敏、金井龍二、河野重行、能村哲郎他, Dictionary

  • 植物細胞培養と有用物質

    遺伝子操作による物質生産, シーエムシー, 2000.05, 作田正明, 駒嶺穆, Research Book

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Papers 【 display / non-display

  • Brassinosteroid-related transcription factor BIL1/BZR1 increases plant resistance to insect feeding

    Biosci. Biotech. Biochem., vol.78(6)(p.960 - 968), 2014.04, Brassinosteroid-related transcription factor BIL1/BZR1 increases plant resistance to insect feeding, Original, Research paper (scientific journal), Coauthor

  • BPG3 is a novel chloroplast protein that involves the greening of leaves and related to brassinosteroid signaling.

    Biosci. Biotechnol. Biochem., vol.78(p.420 - 429), 2014, Yoshizawa, E. Kaizuka, M., Yamagami, A., Higuchi-Takeuchi, M., Matsui, M., Kakei, Y., Shimada, Y., Sakuta, M., Osada, H., Asami, T. and Nakano, T., Original, Research paper (scientific journal), Coauthor

  • Diversity in plant red pigments: anthocyanins and betacyanins.

    Plant Biotechnology Report , vol.8(p.37 - 48), 2014, Sakuta, M, Survey, Research paper (scientific journal), Single Author

  • A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling.

    Planta, vol.237(p.1509 - 1525), 2013, Bekh-Ochir, D., Shimada, S., Yamagami, A., Kanda, S., Ogawa, K., Nakazawa, M.,Matsui, M., Sakuta, M., Osada, H., Asami, T. and Nakano, T., Original, Research paper (scientific journal), Coauthor

  • Effect of phosphate deficiency on the content and biosynthesis of anthocyanins and the expression of related genes in suspension-cultured grape (Vitis sp.) cells

    Plant Physiol. Biochem, 2012.06, Yin Y., Borges, G., Sakuta, M., Crozier, A. and Ashihara H., Original, Research paper (scientific journal), Coauthor

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Published Article 【 display / non-display

  • Why do anthocyanins not occur in the Caryophyllales : Molecular evolution of anthocyanin biosynthesis

    Regulation of Plant Growth & Development, vol.44(p.49 - 55), 2009, Masaaki Sakuta, Survey, Single Author, Accepted, Introduction and explanation (scientific journal)

  • 植物バイオテクノロジーの新たなる挑戦-特集にあたって-

    BIO INDUSTRY, 2005, 作田正明

Presentations 【 display / non-display

  • 新規植物ペプチドホルモンをコードする可能性を持つブラシノステロイド情報伝達因子BIL3の機能発現機構の解析

    吉澤江里子、山上あゆみ、斎藤知恵子、中澤美紀、松井南、作田正明、中野明彦、長田裕之、浅見忠男、中野雄司, Domestic, 2014.03, 日本植物生理学会大会, 富山, 日本植物生理学会, Not Invited, Coauthor

  • BLI1のエンドサイトーシスにおけるブラシノステロイド情報伝達系路上の7回膜貫通タンパク質BIL4の制御機構の解析

    山上あゆみ、斎藤知恵子、中澤美紀、松井南、作田正明、中野明彦、長田裕之、浅見忠男、中野雄司, Domestic, 2014.03, 日本植物生理学会大会, 富山, 日本植物生理学会, Not Invited, Coauthor

  • ナデシコ目植物におけるフラボノイド合成に関わるMYB型転写因子

    田中明日香、岩瀬かおり、原田理紗子、由田和津子、作田正明, Domestic, 2014.03, 日本植物生理学会大会, 富山, 日本植物生理学会, Not Invited, Coauthor

  • ナデシコ目植物のフラボノイド合成に関わるMYB型転写因子の解析

    田中明日香、原田理紗子、岩瀬かおり、由田和津子、作田正明, Domestic, 2013.09, 日本植物学会大会, 札幌, 日本植物学会, Not Invited, Coauthor

  • Comparative Biochemical Studies of DOPA Dioxygenase

    Domestic, 2012.09, 76th Annual Meeting of the Botanical Society of Japan, Himeji, The Botanical Society of Japan, Not Invited, Coauthor

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